|
KMID : 0385520120250010083
|
|
Analytical Science & Technology
2012 Volume.25 No. 1 p.83 ~ p.90
|
|
|
Uncertainty evaluation for the determination of creatinine in urine by LC-MS/MS
|
|
Kim Jin-Young
Kwon Woon-yong
Suh Sung-Ill
In Moon-Kyo
|
|
|
Abstract
|
|
|
The objective of the study was to estimate the measurement uncertainty associated with determination of creatinine (Cr) in urine samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Centrifuged urine samples (10 μL) were diluted with 390 μL of distilled water. To 20 μL aliquots of diluted urine samples, 30 μL of internal standard solution (Cr-d3, 5 μg/mL) and 10 μL of acetonitrile were added and filtered. The samples (1 μL) were introduced into LC-MS/MS with no further pretreatment. Cr was separated on a multi-mode ODS column (Scherzo SM-C18, 75 × 2.0 mm I.D., 3 μm) and quantified by LC-MS/MS operating in MRM mode (Cr, m/z 114.0 → 86.0; Cr-d3, m/z 117.0 → 89.1). The four factors that contribute uncertainty to the final result were extracted and evaluated. The principal factors of contribution to combined
standard uncertainty were sample dilution, calibration curve and repeatability, while the preparation of standard solution was only a minor factor. Relative extended uncertainty of the measured concentration was 14.2% in a real urine sample.
|
|
|
KEYWORD
|
|
|
Measurement uncertainty, urine analysis, creatinine, LC-MS/MS,
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|